Friday, March 26, 2021

O-GlcNAcylation and Its Role in Diseases

Figure 1.  Overview of the Hexosamine Biosynthetic Pathway (HBP) and O-GlcNAcylation.
The HBP integrates four metabolism pathways, including
carbohydrate (glucose), amino acid (glutamine), lipid (Acetyl-CoA) and nucleotide (UTP).


O-linked-N-acetylglucosaminylation (O-GlcNAcylation) is a type of glycosylation that occurs when a monosaccharide, O-GlcNAc, is added onto serine or threonine residues of nuclear or cytoplasmic proteins by O-GlcNAc transferase (OGT) and which can be reversibly removed by O-GlcNAcase (OGA). 

Dysregulation of O-linked β-D-N-acetylglucosamine (O-GlcNAc) has been implicated in many pathologies including Alzheimer's disease (AD), cancer, diabetes, and neurodegenerative disorders.[1]


Figure 2: Dynamic Competition between glycosylation and phosphorylation of proteins.
A: Competition between OGT and kinase for the serine or threonine functional group of a protein.
B: Adjacent-site occupancy where O-GlcNAc and O-phosphatase occur next to each other and
can influence the turnover or function of proteins reciprocally.
The G circle represents an N-acetylglucosamine group, and
the P circle represents a phosphate group. Figure adapted from Hart
.[3]



OGT vs OGA


Since its discovery in the early 1980s, O-GlcNAc addition (O-GlcNAcylation) to serine/threonine residues has been found to be a key post-translational modification of proteins in the nucleus, cytosol and mitochondria. 

As a highly dynamic process, O-GlcNAc rapidly cycles onto serine/threonine residues of target proteins in a fashion analogous to phosphorylation (see Figure 2). 

While there are roughly 500 kinases and 150 phosphatases that regulate protein phosphorylation in humans, there are only 2 enzymes that regulate the cycling of O-GlcNAc:

The chemical reaction of OGT can be written as:

  1. UDP-N-acetyl-D-glucosamine + [protein]-L-serine → UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine
  2. UDP-N-acetyl-D-glucosamine + [protein]-L-threonine → UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine


Using the below pharmacological compounds, you can control the level of protein O-GlcNAcylation:
  • To decrease it, by administering DON and Azaserine
  • To increase it by using OGA blocker
    • Such as Glucosamine, PUGNAc, NButGT
      • NButGT
        • 1,2-dideoxy-2′-propyl-α-d-glucopyranoso-[2,1-D]-Δ2′-thiazoline 
    • Elevated O-GlcNAc has been associated with diabetes.

NButGT (more-specific) vs PUGNAc (less-specific)


The regulation of OGT is directly involved in diabetes. OGT and O-GlcNAc-modified protein levels are increased in the pancreatic islets of diabetic rats.[6] Overexpression of OGT in liver, muscle and fat tissues causes insulin resistance.[7,8] 

Using OGA inhibitors (i.e., PUGNAc and NButGT) can acutely increases the levels of O-GlcNAcylation.  Scientists have noticed that even both inhibitors can induce elevated O-GlcNAcylation, there is a discrepancy found:
  • Treatment with PUGNAc leads to insulin resistance
  • Treatment with NButGT doesn't lead to insulin resistance
This discrepancy might be resulted from the off-target effects (e.g., on lysosomal hexosaminidases) of the less-specific PUGNAc, suggesting the necessity of choosing more specific and selective inhibitors to explore the roles of OGA

Caution should also be made in terms of inhibitor usage, since effects of the inhibitors are, generally, in a dose- and time-dependent manner, which may cause significant differences in protein O-GlcNAcylation levels in a tissue-specific manner and thus the related diabetic phenotypes.

O-GlcNAcylation–Based Treatments as Potential Interventions for AD


O-GlcNAcylation is notably decreased in Alzheimer’s disease (AD) brain. Necroptosis is activated in AD brain and is positively correlated with neuroinflammation and tau pathology. 

In one study,[5] scientists found that NButGT—a specific inhibitor of OGA, reduces production by lowering γ-secretase activity. Moreover, NButGT 
  • Reduces  production 
  • Attenuates
    • Accumulation of Aβ
    • Neuroinflammation
    • Memory impairment 
in the 5XFAD mice. 

This is the first study to show the relationship between Aβ generation and O-GlcNAcylation in vivo. These results suggest that O-GlcNAcylation may be a suitable therapeutic target for the treatment of AD.

O-GlcNAcylation Can Regulate Many Hallmarks of Cancer


Elevated HBP and O-GlcNAcylation has been reported in nearly all cancers examined and can regulate many “hallmarks of cancer”, including growth, survival, metabolism, angiogenesis, and metastasis.[12]

Up to this point, nearly all evidence suggests that the HBP helps fuel cancer cell metabolism, growth, survival, and spread. Further research should elucidate whether the HBP plays a role in cancer initiation and maintenance, heterogeneity, and regulation of the tumor microenvironment, including immune surveillance.[13]

References

  1. Hart, Gerald W.; Slawson, Chad; Ramirez-Correa, Genaro; Lagerlof, Olof (2011-06-07). "Cross Talk Between O-GlcNAcylation and Phosphorylation: Roles in Signaling, Transcription, and Chronic Disease". Annual Review of Biochemistry. 80: 825–858.
  2. Haltiwanger, RS; Holt, GD; Hart, GW (1990-02-15). "Enzymatic Addition of O-GlcNAc to Nuclear and Cytoplasmic Proteins. Identification of a Uridine diphospho-N-acetylglucosamine:peptide beta-N-acetylglucosaminyltransferase". Journal of Biological Chemistry. 265 (5): 2563–8.
  3. Hart GW, Housley MP, Slawson C (April 2007). "Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins". Nature. 446 (7139): 1017–22.
  4. Macauley, MS; Bubb, AK; Martinez-Fleites, C; Davies, GJ; Vocadlo, DJ (2008-12-12). "Elevation of Global O-GlcNAc Levels in 3T3-L1 Adipocytes by Selective Inhibition of O-GlcNAcase Does Not Induce Insulin Resistance". The Journal of Biological Chemistry. 283 (50): 34687–95.
  5. O-GLCNACYLATION AMELIORATES THE PATHOLOGICAL MANIFESTATIONS OF ALZHEIMER’S DISEASE BY INHIBITING NECROPTOSIS
  6. Akimoto Y, Hart GW, Wells L, et al. Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki rats. Glycobiology. 2007;17:127–140.
  7. McClain DA, Lubas WA, Cooksey RC, et al. Altered glycan-dependent signaling induces insulin resistance and hyper-leptinemia. Proc Natl Acad Sci USA. 2002;99:10695–10699.
  8. Yang X, Ongusaha P, Miles P, et al. Phosphoinositide signaling links O-GlcNAc transferase to insulin resistance. Nature. 2008;451:964–970.
  9. Macauley MS, Bubb AK, Martinez-Fleites C, et al. Elevation of global O-GlcNAc levels in 3T3-L1 adipocytes by selective inhibition of O-GlcNAcase does not induce insulin resistance. J Biol Chem. 2008;283:34687–34695.
  10. Macauley MS, He Y, Gloster TM, et al. Inhibition of O-GlcNAcase using a potent and cell-permeable inhibitor does not induce insulin resistance in 3T3-L1 adipocytes. Chem Biol. 2010;17:937–948.
  11. Macauley MS, Shan X, Yuzwa SA, et al. Elevation of global O-GlcNAc in rodents using a selective O-GlcNAcase inhibitor does not cause insulin resistance or perturb glucohomeostasis. Chem Biol. 2010;17:949–958.
  12. Ferrer CM, Sodi VL, Reginato MJ. O-GlcNAcylation in cancer biology: linking metabolism and signaling. J Mol Biol. 2016;428:3282–94.
  13. Fueling the fire: emerging role of the hexosamine biosynthetic pathway in cancer

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